Chlortetracycline Elisa Test Kit
Chlortetracycline Elisa Test Kit
Product Description REAGEN™Chlortetracycline ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Chlortetracycline in hony, milk, meat and urine. The unique features of the kit are:
Kit Contents, Storage and Shelf Life
REAGEN™ Chlortetracycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
|
Kit Contents |
Amount |
Storage |
|
Chlortetracycline-coated Microtiter Plate |
1x 96-well plate(8wells x12 strips) |
2-8°C |
|
Chlortetracycline Standards stock (450ng powder) Empty Vials for standards: Negative control (white cap tube) 0.05 ng/mL (yellow cap tube) 0.15ng/mL (orange cap tube) 0.5 ng/mL (pink cap tube) 1.5 ng/mL (purple cap tube) 4.5 ng/mL (blue cap tube) |
3 1 1 1 1 1 1 |
-20°C 2-8°C |
|
|
|
|
|
Chlortetracycline Antibody #1 |
3xtubes |
2-8°C * |
|
Antibody #1 diluent |
15ml |
2-8°C |
|
100X HRP-Conjugated Antibody #2 |
0.3mL |
2-8°C * |
|
Antibody #2 Diluent ** |
20 mL |
2-8°C |
|
20X Wash Solution ** |
28 mL |
2-8°C |
|
Stop Buffer ** |
20 mL |
2-8°C |
|
TMB Substrate ** |
12 mL |
2-8°C |
|
20Xtet meat extration buffer |
20mL |
2-8°C |
* If you are not planning to use the kit for over 3 months, store Chlortetracycline Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity
|
Sample Type |
Detection Limit (ng/g or ppb) |
|
Honey |
2.5 |
|
Meat |
10 |
|
Milk |
10 |
|
Milk powder |
45 |
|
Serum |
2 |
|
Urine |
2 |
Specificity
|
Analytes |
Cross-Reactivity (%) |
|
Chlortetracycline |
100 |
|
Oxytetracycline |
91 |
|
Minocycline |
73 |
|
Demeclocycine |
39 |
|
Tetracycline |
119 |
|
Doxycycline |
60 |
